Biographical Info

Dr. Aleksunes is Professor in the Rutgers University, Ernest Mario School of Pharmacy, Department of Pharmacology and Toxicology, Project Lead of the NJ Alliance for Clinical and Translational Science CTSA Workforce Development Core, and Director of the Joint Graduate Program in Toxicology and the Summer Undergraduate Research Fellowship. Dr. Aleksunes is also a member of the CounterAct Research Center of Excellence where she Co-Leads the Research Education Core and Pharmacology and Drug Development Core. Within the NIEHS P30 Center for Environmental Exposures and Disease, Dr. Aleksunes is Co-Director of the Environmental/Chemical Pathology Core and Co-Director of Career Development and Mentoring.

Research Areas

Drugs and chemicals are often too large to enter and exit cells unassisted. Transporter proteins can be used as gates that regulate which chemicals leave cells. In fact, the ATP-binding cassette transporters (ABC) are members of a superfamily of transporters found in the plasma membrane. ABC transporters function as efflux pumps that remove chemicals from the cell. Our laboratory studies members of three families of efflux transporters: ABCB1 (Multidrug resistance protein 1, MDR1, P-glycoprotein), ABCC1-6 (Multidrug resistance-associated proteins, MRP), and ABCG2 (Breast cancer resistance protein, BCRP). These transporters are important in removing chemicals from the liver and kidneys and can protect against target organ toxicity. Similarly, these efflux pumps are expressed in the placenta and participate in maternal-fetal xenobiotic disposition, thereby protecting the developing fetus from toxicant exposure.

Dr. Aleksunes’ laboratory investigates how drug transporters in the liver, kidneys, brain, and placenta protect against the accumulation and toxicity of pharmaceuticals and environmental chemicals. Her studies aim to 1) characterize substrates and inhibitors for efflux transporters, 2) determine how genetic variants influence transporter function, and 3) understand the regulatory mechanisms that control expression of transporters. Dr. Aleksunes’ laboratory utilizes molecular biology, cell biology, in vivo transgenic animal, explant human tissue, biomarkers of toxicity, and pharmacokinetic approaches to study the interplay of drug transport and toxicology.

Dr. Aleksunes and her laboratory are funded by NIH R01ES021800, R01ES029275, R01GM123330, and F31ES029794  Dr. Aleksunes is Principal Investigator for T32ES007148 and R25ES020721 and Intern Programs from the Society of Toxicology and the American Society for Pharmacology and Experimental Therapeutics. She also participates in U54TR002258 and U54AR055073.

Research Highlights

The Aleksunes laboratory includes research scientists (Xia Wen), graduate students (Ludwik Gorczyca, Danielle Kozlosky, and Maxine Abustan), postdoctoral fellows (Ranran Zhang and Lauren Walker), and undergraduate students (Fatimah Mosaad, Brian Rinelli, Chris Pella, Duaa Eisa, Hye Min Shin, Perihan Badawy, Rebecca Sun, and Victoria Woo). Students from China Pharmaceutical University and Sun Yat-Sen University spend rotations in the Aleksunes laboratory as well.

Highlights from the research team include:

• Identification of pharmacogenetic risk factors, drug interactions, and biomarkers for cisplatin-induced nephrotoxicity in humans.
• Investigation of enterohepatic bile acid signaling and homeostasis during pregnancy.
• Characterization of environmental chemicals as substrates and inhibitors of human placental efflux transporters.
• Elucidation of regulatory mechanisms controlling transporter expression in the placenta.
• Characterization of cell-cell communication between microglia and brain endothelial cells.
• Identification of transporters in the blood-brain barrier that regulate exposure to pesticides.
• Screening of diverse chemical sets to inform the development and refinement of virtual Adverse Outcome Pathways (vAOPs).

Scholarly Activities

• Aleksunes is a Grant Writing Mentor in the Big 10 National Research Mentoring Network.
• Program Director of the ASPET Summer Research Fellowship Program at Rutgers University, Ernest Mario School of Pharmacy.
• Organizing Committee for Pharmacy Research Day at Rutgers University, Ernest Mario School of Pharmacy.
• Member of the Rutgers Cancer Institute of New Jersey
• Vice Chair (2021) and Chair (2023) of the Multi-Drug Efflux Systems Gordon Research Conference
• Vice Chair-Elect (2019-2020) of the Mid-Atlantic Society of Toxicology
• Member and Chair (2015-2016) of the Steering Committee for the New Jersey Drug Metabolism Discussion Group.
• Aleksunes published an extensive review of drug transporter function and regulation in Pharmacological Reviews (2010) 62:1-96.
• Elected to the Drug Transport Focus Group of American Association of Pharmaceutical Scientists.

In the News

• In 2019, Dr. Aleksunes was awarded the Richard Okita Early Career Award in Drug Metabolism and Disposition (American Society for Pharmacology and Experimental Therapeutics) and Outstanding Young Investigator Award (Women in Toxicology, Society of Toxicology).
• Working with Dr. Hao Zhu at Rutgers-Camden, their team is advancing the screening and prediction of chemical toxicities.
• Dr. Aleksunes was awarded the 2018 Award of Merit from the Lambda Kappa Sigma Professional Pharmacy Fraternity.
• NIEHS profiles the exciting placental research of Dr. Aleksunes.
• Dr. Aleksunes assisted Dr. Dan Huh in his team’s development of a Placenta-on-a-Chip at the University of Pennsylvania.
• Find out about medications and natural products that can damage the liver with Dr. Aleksunes
• Aleksunes was awarded the 2016 Achievement Award from the Society of Toxicology
• Learn more about becoming a Medical Researcher with Dr. Aleksunes in a recent Youtube video!
• In 2015, Dr. Aleksunes was awarded the Mentor of the Year from AFPE, a Global Scientific Achievement Award from Xenotech and the Tradition of Excellence Award from the UConn Pharmacy Alumni Association.
• Dr. Aleksunes became a Diplomate of the American Board of Toxicology in 2012.
• NIEHS announced the 2012 Outstanding New Environmental Scientists awardees, including Dr. Aleksunes.

Recent Publications

1. Chung, E, Russo, DP, Ciallella, HL, Wang, YT, Wu, M, Aleksunes, LM, Zhu, H. Data-Driven Quantitative Structure-Activity Relationship Modeling for Human Carcinogenicity by Chronic Oral Exposure. Environ Sci Technol. 2023;57 (16):6573-6588. doi: 10.1021/acs.est.3c00648. PubMed PMID:37040559 PubMed Central PMC10134506
2. Barrett, ES, Rivera-Núñez, Z, Getz, K, Ohman-Strickland, P, Zhang, R, Kozlosky, D, Doherty, CL, Buckley, BT, Brunner, J, Miller, RK et al.. Protective role of the placental efflux transporter BCRP/ABCG2 in the relationship between prenatal cadmium exposure, placenta weight, and size at birth. Environ Res. 2023;225 :115597. doi: 10.1016/j.envres.2023.115597. PubMed PMID:36863650 PubMed Central PMC10091184
3. Meakin, C, Kim, C, Lampert, T, Aleksunes, LM. High-throughput screening of toxicants that modulate extravillous trophoblast migration. Toxicol Lett. 2023;375 :1-7. doi: 10.1016/j.toxlet.2022.12.004. PubMed PMID:36535517 PubMed Central PMC9877196
4. Jia, X, Wen, X, Russo, DP, Aleksunes, LM, Zhu, H. Mechanism-driven modeling of chemical hepatotoxicity using structural alerts and an in vitro screening assay. J Hazard Mater. 2022;436 :129193. doi: 10.1016/j.jhazmat.2022.129193. PubMed PMID:35739723 PubMed Central PMC9262097
5. Hafey, MJ, Aleksunes, LM, Bridges, CC, Brouwer, KR, Chien, HC, Leslie, EM, Hu, S, Li, Y, Shen, J, Sparreboom, A et al.. Transporters and Toxicity: Insights From the International Transporter Consortium Workshop 4. Clin Pharmacol Ther. 2022;112 (3):527-539. doi: 10.1002/cpt.2638. PubMed PMID:35546260
6. George, B, Szilagyi, JT, Joy, MS, Aleksunes, LM. Regulation of renal calbindin expression during cisplatin-induced kidney injury. J Biochem Mol Toxicol. 2022;36 (7):e23068. doi: 10.1002/jbt.23068. PubMed PMID:35403300 PubMed Central PMC9296602
7. Memon, N, Lee, CW, Herdt, A, Weinberger, BI, Hegyi, T, Carayannopoulos, MO, Aleksunes, LM, Guo, GL, Griffin, IJ. Suppression of Bile Acid Synthesis in a Preterm Infant Receiving Prolonged Parenteral Nutrition. J Clin Exp Hepatol. 2022;12 (1):200-203. doi: 10.1016/j.jceh.2021.04.007. PubMed PMID:35068799 PubMed Central PMC8766543
8. Kozlosky, D, Barrett, E, Aleksunes, LM. Regulation of Placental Efflux Transporters during Pregnancy Complications. Drug Metab Dispos. 2022;50 (10):1364-1375. doi: 10.1124/dmd.121.000449. PubMed PMID:34992073 PubMed Central PMC9513846
9. Meakin, C, Barrett, ES, Aleksunes, LM. Extravillous trophoblast migration and invasion: Impact of environmental chemicals and pharmaceuticals. Reprod Toxicol. 2022;107 :60-68. doi: 10.1016/j.reprotox.2021.11.008. PubMed PMID:34838982 PubMed Central PMC8760155
10. Ciallella, HL, Russo, DP, Aleksunes, LM, Grimm, FA, Zhu, H. Revealing Adverse Outcome Pathways from Public High-Throughput Screening Data to Evaluate New Toxicants by a Knowledge-Based Deep Neural Network Approach. Environ Sci Technol. 2021;55 (15):10875-10887. doi: 10.1021/acs.est.1c02656. PubMed PMID:34304572 PubMed Central PMC8713073