Stereology Description

The Molecular Histology Center (MHC) performs design-based stereology. This is a statistically-based procedure for estimating a variety of three dimensional properties using two-dimensional images, usually from a series of thinly sectioned tissue sections. Estimates that can be performed using this technique include volume of a structure, surface of a structure, boundary length, population number, density, and volume or area of specific particles. Designed-based stereology does not make any assumptions about the size or shape of a structure being studied. As such, the estimations are unbiased and precise. The application of a stereological procedure to estimate a quantity is known as a probe. The MHC performs a variety of probes as listed under "Services". The MHC utilizes the software application Stereo Investigator by Microbrightfield Incorporated (Williston, Vermont). This software is used in conjunction with a variety of hardware including an AX 70 Provis microscope with fluorescence capability including rexba fluorescence shading, a LEP XYZ stage controller, a Heidenhain Z-axis ??, and an Optronix color video camera.

The technique of unbiased stereology requires both specialized equipment as well as expertise in the application and design of probes. The MHC will assist in the application of these techniques using your tissue to answer your question of interest. This is not a technique that an individual would care to set up unless it will be used routinely. The MHC routinely performs stereology and has the experience, reagents, and equipment to efficiently apply this technique.

The cost of stereology will depend on a variety of factors including the number of samples for which the probe will be performed, the amount of tissue sectioning and staining required to prepare the tissue for analysis, and the number of probes and counts that must be obtained to achieve statistical reliability. In addition, before actual experimental data can be obtained, a preliminary study must be done to test and confirm all of the assumptions for staining and counting. With the use of a worksheet we can assist with generating a cost estimate for your project. Services provided include consultation about your experiment, tissue acquisition, tissue sectioning and staining, and performance of stereologic probes. Our specific services are reviewed on the "Services" page.

Specific methods for various techniques can be downloaded for use with grant applications and publications from the stereology home page.

Molecular Histology Center Environmental and Occupational Health Sciences Institute, 170 Frelinghuysen Road, Piscataway, NJ 08854 Phone: 732-445-3729 For additional information contact ekr@eohsi.rutgers.edu

Updated on Friday, June 03, 2005