In Situ Hybridization

Housekeeping Genes

Gene	Species	Tm	Inc     Temp Range    (C)	Wash Temp Range    (C)	Film Exp Range (days)	Anti-sense Sequence	Complementary to Sequences	Length
GAPDH	mouse	49.1	33-38	35-41	5-9	gtgccgttgaatttgccgtgagtggagtcatactggaacatgtagacc	169-216	48
ELF2	mouse	48.3	33-38	38-41	6-10	cccatctcagcagcctccttctcaaacttttcgatggttcgcttgtcg	141-188	48
cyclophilin	mouse	49.9	32-35	35-41	5-10	tctttggaactttgtctgcaaacagctcgaaggagacgcggcccaagg	88-135	48
transferrin receptor	mouse	47.4	32-38	35-41	7-20	ctgacttgtctgtctcctccgtttcagccagtttcacacactcctctt	284-331	48
betaActin	mouse	54.2	35-41	37-43	3-7	tcacccacataggagtccttctgacccattcccaccatcacaccttgg	42-89	48

Molecular Histology Center Environmental and Occupational Health Sciences Institute, 170 Frelinghuysen Road, Piscataway, NJ 08854 Phone: 732-445-3729 For additional information contact ekr@eohsi.rutgers.edu

Updated on Friday, June 03, 2005