In Situ Hybridization

The Molecular Histology Center (MHC) performs both isotopic and nonisotopic in situ hybridization histochemistry. This is a method for detecting the expression of mRNA in tissue sections. Both quantitative and qualitative analyses can be performed. Two types of probes can be used, short DNA oligonucleotides (typically 48 bp) or longer length RNA or DNA probes. The choice of optimal probe and detection method are made based on the needs of an experiment.

The technique of in situ hybridization histochemistry demands rigor and commitment to perform well. It is not a technique one would necessarily want to implement for a single experiment or a small set of experiments. The MHC performs this technique routinely and has the experience, reagents, and equipment to efficiently apply this technique. However, with all molecular and tissue-based assays testing and development are required. The MHC has a series of assurances and quality control steps that assist in the process of assay development including testing the quality of probe labeling and tissue.

The cost of in situ hybridization is based on the type of the probe used, the number of probes examined, the number of conditions and testing required, the number of slides, and choice of detection method and analyses. With the use of a worksheet we can assist with generating a cost estimate for your project. Services provided include consultation about your experiment, oligonucleotide design, oligonucleotide purchase, oligonucleotide dissolving at stock and working concentrations, tissue acquisition and sectioning, probe labeling, purification, and determination of specific activity, tissue hybridization, film or phosphor screen exposure, densitometry, emulsion dipping, grain counting, and image capture. Our specific services are reviewed on Services Page.

Specific methods for various techniques can be downloaded for use with grant applications or publications.

Molecular Histology Center Environmental and Occupational Health Sciences Institute, 170 Frelinghuysen Road, Piscataway, NJ 08854 Phone: 732-445-3729 For additional information contact ekr@eohsi.rutgers.edu

Updated on Friday, June 03, 2005