Immunohistochemistry Description

 

The Molecular Histology Center (MHC) performs chromogenic and fluorescence immunohistochemistry (IHC). Typically, these assays are done as a prelude to stereology by the MHC. However, for investigators who do not have experience with immunohistochemistry, we can develop or perform assays with your antibody and tissue of interest. Users can select either chromogenic visualization using DAB as the chromogen and a counterstain such as cresyl violet or fluorescence detection using secondary antibodies labeled with a variety of fluorophores and DAPI as a nuclei counterstain. Fluorescence labeled secondaries of choice include the Alexa series of fluorophores. Assays previously established and published in the literature can be used or modified or novel assays can be developed.

Immunohistochemistry is a widespread technique with many variations in its performance. Typically, we use free-floating sections and perform a variety of incubations using different concentrations of primary and secondary antibodies to optimize the labeling. Tissue preparation can strongly influence specific and nonspecific labeling. We can provide a variety of tissues prepared in a variety of manners for testing and analysis. We perform all sectioning, mounting, and assays. We are also capable of photographic image processing and other studies.

We will generate a description of the specific method used for each antibody and assay which will be available for grant applications or publications.

Molecular Histology Center Environmental and Occupational Health Sciences Institute, 170 Frelinghuysen Road, Piscataway, NJ 08854 Phone: 732-445-3729 For additional information contact ekr@eohsi.rutgers.edu

Updated on Friday, June 03, 2005