Molecular
Toxicology Sring 2008
(11:115:434/16:963:633)
Meeting
times:
Molecular Toxicology
Tuesday 3:00-4:30PM EOHSI
Conf
Room C
Goals
of the Course: The goal of this
course is to acquaint students with the current laboratory techniques
used in
toxicological research. By the end of
the 15-week period, students should be able to analyze research data
and
evaluate the use of the techniques that are covered in the course.
Student
performance in the Molecular Toxicology
lecture section (11:115:434/ 16:963:633) will be evaluated based on two
take
home examinations.
Course
Contacts:
Molecular
Toxicology (115:434/16:963:633)
Dr.
Renping Zhou
Email: rzhou@rci.rutgers.edu
Phone: (732) 445-3400 X264
Dr.
Jun-Yan Hong
Email:
jyhong@eohsi.rutgers.edu
Phone:
(732) 235-2845
Dr.
Lori White
Email: lawhite@aesop.rutgers.edu
Phone: 732-932-9763 X128
Syllabus
| Date |
Topic |
Lecturer |
| 1/22 |
Organizational meeting |
|
| 1/29 |
DNA Cloning and expression of neuroregulatory genes and techniques will be discussed using both in vitro and in vivo analysis. |
Dr. Zhou |
| 2/5 |
The general principles of tissue culture will be presented along with neurostem cell culture techniques. | Dr. Woodbury |
| 2/12 |
Lecture:
Cell isolation using flow cytometry
acrobat 
Powerpoint 
acrobat Powerpoint
|
Dr. V. Sunil |
| 2/19 |
Analysis of gene expression (northern blot, Rnase protection and SiRNA) | Dr. Chen |
| 2/26 |
Analysis
of gene expression using PCR acrobat
|
Dr. White |
| 3/4 |
The concepts of gene chips and cDNA array analysis will be discussed using the investigation of neuroplasticity as a model. | Drs. Alder & Thakker-Varia |
| 3/11 |
The role of imaging techniques in analysis of cell adhesion and neurotoxicity will be discussed. | Dr. Reuhl |
| 3/15-3/23 |
Spring Break/SOT Meeting |
|
| 3/25 |
Protein-Protein interaction: yeast-two-hybrid analysis | Dr. Zhou |
| 4/1 |
Analysis of biological activity of proteins. Detection of biological activity of proteins will be discussed as they apply to neurotrophism and axonal guidance | Dr. Meiners |
| 4/8 |
Ecotopic gene expression using transgenic mice and gene inactivation using knockout technologie | Dr. Hong |
| 4/15 |
The
lecture will present an overview of chromatographic techniques and
detection methods used in current methods. The use
of UV, fluorescence, radiation, and mass spectral analysis techniques
in current methods will be discussed. acrobat Powerpoint |
Dr. Buckley |
| 4/22 |
Alternative model systems from fish to insects. | Dr. White |
| 4/29 |
Proteomics and human diseases | Dr. Lobel |